Chèvre anti-Cobaye IgG (F(ab')2 Region) Anticorps (Alkaline Phosphatase (AP))

N° du produit ABIN101307

N° du produit (Fournisseur): 606-1504

Aperçu rapide pour Chèvre anti-Cobaye IgG (F(ab')2 Region) Anticorps (Alkaline Phosphatase (AP)) (ABIN101307)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: F(ab')2 Region

Reactivité: Cobaye

Hôte: Chèvre

N° du produit (Fournisseur): 606-1504

Fournisseur: Rockland

Fonction: Guinea Pig IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Guinea Pig IgG, Guinea Pig IgG F(ab')2 and Guinea Pig Serum.  No reaction was observed against Guinea Pig IgG F(c).

Attributs du produit: Anti-Guinea Pig IgG F(c) Alkaline Phosphatase generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène: Optional[Immunogen]: Guinea Pig IgG F(ab')2 fragment

Information d'application

Indications d'application: Application Note: Anti-Guinea Pig IgG F(ab')2 Alkaline Phosphatase Antibody has been tested by ELISA. This product has been assayed against 1.0 μg of Guinea Pig IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.  Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:15,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.1 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Anti-Guinea Pig IgG F(ab')2 Alkaline Phosphatase antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Guinea Pig IgG F(c) will not bind Guinea Pig IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.